Western Blot Stripping Buffer (ab270550) is a robust, ready-to-use solution allowing primary and secondary antibodies to be quickly removed from nitrocellulose and PVDF membranes and, as a result, Western blots to be reprobed with different antibodies. This permits more time-efficient experiments that use less sample by reusing membrane without having to rerun gels and blots. Using a PVDF membrane will minimize the loss of sample protein when stripping.
This product is manufactured by Expedeon, an Abcam company. Expedeon product code ECLB0500 was previously called Western Blot Stripping Buffer – 500ml and is the same as the 500 mL size of this product. Expedeon product code ECLB5000 was previously called Western Blot Stripping Buffer – 5L and is the same as the 5000 mL size.
The 500mL size is provided as 2 x 250 mL bottles.
Properties
Form: Liquid
Storage Instructions: Shipped at Room Temperature. Store at Room Temperature. Store at +4°C.
Storage buffer: Constituent: 1.5% Hydrochloric acid
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end-user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay-dependent concentration.
|
Note: All products are “FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES” For licensing inquiries, contact [email protected]