total rna purification kit

Product Description:

Our Coronavirus SARS-CoV-2 RNA Extraction Kit/RNA Isolation Kit offers simple solutions for RNA isolation. Our kit is designed to extract and purify RNA. Recovered RNA is compatible with COVID-19 RT-PCR Testing. We use advanced silica bind and elute technology for pure RNA extraction. Maintain Purity and Integrity of Extracted RNACompatible with all COVID-19 RTPCR KitsExtraction in a high quantity of Total RNAMany Sample types supported including cells, blood, tissue, and FFPE tissues

Principle

MDS Viral RNA Extraction Kit/RNA Isolation Kit represents a well-established technology for general-use viral RNA preparation. The kit combines the selective binding properties of a silica-based membrane with the speed of the micro spin and is highly suited for simultaneous processing of multiple samples. The sample is first lysed under highly denaturing conditions to inactivate RNases and to ensure isolation of intact viral RNA.

Buffering conditions are then adjusted to provide optimum binding of the RNA to the membrane, and the sample is loaded onto the spin column. The RNA binds to the membrane, and contaminants are efficiently washed away in 2 steps using 2 different wash buffers. High-quality RNA is eluted in a special RNase-free buffer, ready for direct use or safe storage. The purified RNA is free of protein, nucleases, and other contaminants and inhibitors. The special membrane guarantees the extremely high recovery of pure, intact RNA in just 20 min without the use of phenol/chloroform extraction or alcohol precipitation.

Shipping and storage

Spin columns should be stored dry at room temperature (15–25°C); storage at higher temperatures should be avoided. All solutions should be stored at room temperature unless otherwise stated. Under these conditions, spin columns and all buffers and reagents can be stored until the expiration date on the kit box, without showing any reduction in performance

Intended Use

MDS Viral RNA Extraction Kit/RNA Isolation Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Notes:

  • Ethanol is not supplied with the kit
  • Mix Carrier RNA (Lyophilised Form) with Carrier RNA Solubilization Buffer to prepare Carrier RNA solution. Step applicable only if carrier RNA is provided as Lyophilised form
  • If your RT PCR KIT is supplied with an exogenous internal control (IC), then the EIC supplied with your RT PCR kit has to be added in step 3 as per the chart below.
  • If your RT PCR KIT is supplied with an endogenous internal control , then the IC supplied with your RT PCR kit need not be added in step 3 as per the chart below.

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