De opkomende regulerende rollen van niet-coderende RNA’s

De opkomende regulerende rollen van niet-coderende RNA’s in de immuunfunctie van vissen: MicroRNA’s versus lange niet-coderende RNA’s 

The genome might be thought of as uncooked knowledge expressed in proteins and numerous sorts of noncoding RNAs (ncRNAs). Nonetheless, a big portion of the genome is devoted to ncRNAs, which in flip symbolize a substantial quantity of the transcriptome. ncRNAs are modulated on ranges of sort and quantity each time any physiological course of happens or as a response to exterior modulators. ncRNAs, usually forming complexes with different companions, are key molecules that affect numerous mobile processes. Based mostly on the information of mammalian biology, ncRNAs are identified to control and management numerous trafficking pathways and mobile actions.

Lengthy noncoding RNAs (lncRNAs) notably have numerous and extra regulatory roles than microRNAs. Increasing these research on fish has derived the identical conclusion with relevance to different species, together with invertebrates, explored the potentials to harness such sorts of RNA to additional perceive the biology of such organisms, and opened gates for making use of latest applied sciences, equivalent to RNA interference and delivering micromolecules as microRNAs to residing cells and presumably to focus on organs. These applied sciences ought to enhance aquaculture productiveness and fish well being, in addition to assist perceive fish biology.

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blsystems

Anti-Human BDNF Antibody

102-P02 100 µg
EUR 245.7
Description: BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0 kDa homodimer of two 119 amino acid subunits linked by strong non-covalent interactions. Human and Mouse BDNF sequences are identical.

Anti-Human BDNF Antibody

101-M138 100 µg
EUR 399
Description: BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0 kDa homodimer of two 119 amino acid subunits linked by strong non-covalent interactions. Human and Mouse BDNF sequences are identical.

Anti-Human BDNF Antibody

101-M139 100 µg
EUR 399
Description: BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0 kDa homodimer of two 119 amino acid subunits linked by strong non-covalent interactions. Human and Mouse BDNF sequences are identical.

Anti-Human BDNF Antibody

101-M229 100 µg
EUR 399
Description: Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family comprises at least four proteins including NGF, BDNF, NT3, and NT4/ 5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a noncovalently linked homodimer. BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye, and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung, and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.

anti- BDNF antibody

FNab00859 100µg
EUR 606.3
Description: Antibody raised against BDNF

anti- BDNF antibody

FNab10014 100µg
EUR 658.5
Description: Antibody raised against BDNF

Antibody for Human BDNF

SPC-703D-A565 0.1mg
EUR 480
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to ATTO 565.

Antibody for Human BDNF

SPC-703D-A633 0.1mg
EUR 480
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to ATTO 633.

Antibody for Human BDNF

SPC-703D-A655 0.1mg
EUR 480
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to ATTO 655.

Antibody for Human BDNF

SPC-703D-A680 0.1mg
EUR 480
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to ATTO 680.

Antibody for Human BDNF

SPC-703D-A700 0.1mg
EUR 480
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to ATTO 700.

Antibody for Human BDNF

SPC-703D-APCCY7 0.1mg
EUR 565.2
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to APC/Cy7.

Antibody for Human BDNF

SPC-703D-DY350 0.1mg
EUR 496.8
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to Dylight 350.

Antibody for Human BDNF

SPC-703D-DY405 0.1mg
EUR 483.6
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to Dylight 405.

Antibody for Human BDNF

SPC-703D-DY488 0.1mg
EUR 471.6
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to Dylight 488.

Antibody for Human BDNF

SPC-703D-DY594 0.1mg
EUR 474
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to Dylight 594.

Antibody for Human BDNF

SPC-703D-DY633 0.1mg
EUR 468
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to Dylight 633.

Antibody for Human BDNF

SPC-703D-P594 0.1mg
EUR 488.4
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to PE/ATTO 594.

Antibody for Human BDNF

SPC-703D-STR 0.1mg
EUR 477.6
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to Streptavidin.

Rabbit Anti Human BDNF Monoclonal Clone BEH-2

IRBAHUBDNFBEH2C100UL each
EUR 496
Description: Rabbit Anti Human BDNF Monoclonal Clone BEH-2

RECOMBINANT HUMAN BDNF

GWB-DE906C 0.01 mg Ask for price

Recombinant Human BDNF

P0449 100ug
EUR 626.83
Description: Recombinant Human protein for BDNF

Recombinant Human BDNF

Z100065 10 µg
EUR 235
Description: BDNF is found in neurons of the central nervous system. It is expressed predominantly in hippocampus, cortex, and synapses of the basal forebrain. The biological activity of BDNF is mediated by a receptor that belongs to the trk family of receptors encoding a tyrosine-specific protein kinase. BDNF only binds weakly to the gp140trk receptor (to which NGF binds with high affinity), and it binds to the NGF receptor known as LNGFR. BDNF selectively supports the survival of primary sensory neurons and retinal ganglia. The factor supports survival and differentiation of certain cholinergic neurons and also some dopaminergic neurons in vitro. BDNF does not appear to act on sympathetic ganglia. In specific neurons of the central nervous system located in the hippocampus and the cortex the synthesis of BDNF is influenced by neuronal activity either positively (glutamate transmitter system) or negatively (GABA transmitter system).

Recombinant Human BDNF

Z100069 1.0 mg
EUR 6900
Description: BDNF is found in neurons of the central nervous system. It is expressed predominantly in hippocampus, cortex, and synapses of the basal forebrain. The biological activity of BDNF is mediated by a receptor that belongs to the trk family of receptors encoding a tyrosine-specific protein kinase. BDNF only binds weakly to the gp140trk receptor (to which NGF binds with high affinity), and it binds to the NGF receptor known as LNGFR. BDNF selectively supports the survival of primary sensory neurons and retinal ganglia. The factor supports survival and differentiation of certain cholinergic neurons and also some dopaminergic neurons in vitro. BDNF does not appear to act on sympathetic ganglia. In specific neurons of the central nervous system located in the hippocampus and the cortex the synthesis of BDNF is influenced by neuronal activity either positively (glutamate transmitter system) or negatively (GABA transmitter system).

Mouse Monoclonal anti-Human BDNF

hAP-0109 100ug
EUR 250

Mouse Monoclonal anti-Human BDNF

hAP-0109A 100ug
EUR 250

Mouse Monoclonal anti-Human BDNF

hAP-0109B 100ug
EUR 250

Rabbit Anti-Human BDNF Polyclonal

SPC-703D 0.1mg
EUR 331
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is unconjugated.

Rabbit Anti-Human BDNF Polyclonal

SPC-703D-A390 0.1mg
EUR 385
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to ATTO 390.

Rabbit Anti-Human BDNF Polyclonal

SPC-703D-A488 0.1mg
EUR 384
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to ATTO 488.

Rabbit Anti-Human BDNF Polyclonal

SPC-703D-A594 0.1mg
EUR 384
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to ATTO 594.

Rabbit Anti-Human BDNF Polyclonal

SPC-703D-ALP 0.1mg
EUR 377
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to Alkaline Phosphatase.

Rabbit Anti-Human BDNF Polyclonal

SPC-703D-APC 0.1mg
EUR 383
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to APC .

Rabbit Anti-Human BDNF Polyclonal

SPC-703D-BI 0.1mg
EUR 380
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to Biotin.

Rabbit Anti-Human BDNF Polyclonal

SPC-703D-FITC 0.1mg
EUR 375
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to FITC.

Rabbit Anti-Human BDNF Polyclonal

SPC-703D-HRP 0.1mg
EUR 371
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to HRP.

Rabbit Anti-Human BDNF Polyclonal

SPC-703D-PCP 0.1mg
EUR 383
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to PerCP.

Rabbit Anti-Human BDNF Polyclonal

SPC-703D-RPE 0.1mg
EUR 381
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is conjugated to RPE .

Rabbit Anti-Human BDNF Polyclonal

SPC-703S 0.012mg
EUR 44
Description: A polyclonal antibody for BDNF from Human | Mouse . The antibody is produced in rabbit after immunization with human synthetic peptide from the N-terminal of human BDNF. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This BDNF antibody is unconjugated.

Anti-rh BDNF Antibody

Y075081 100 µl
EUR 535

BDNF, human recombinant

4004-10 each
EUR 333.6

BDNF, human recombinant

4004-1000 each
EUR 6571.2

BDNF, human recombinant

4004-250 each
EUR 2461.2

BDNF, human recombinant

4004-50 each
EUR 816

BDNF, human recombinant

P1007-.01 10 µg
EUR 261
Description: BDNF, expressed in E. coli, a potent neurotrophic factor.

BDNF, human recombinant

P1007-.05 50 µg
EUR 658
Description: BDNF, expressed in E. coli, a potent neurotrophic factor.

BDNF, human recombinant

P1007-.25 250 µg
EUR 2050
Description: BDNF, expressed in E. coli, a potent neurotrophic factor.

BDNF, human recombinant

P1007-1 1 mg
EUR 5530
Description: BDNF, expressed in E. coli, a potent neurotrophic factor.

Anti-pro BDNF antibody

STJ13100047 100 µl
EUR 512.4

Anti-pro BDNF antibody

STJ13100048 500 µg
EUR 512.4

Anti-pro BDNF antibody

STJ13100063 100 µl
EUR 512.4

Anti-mature BDNF antibody

STJ13100056 100 µl
EUR 512.4

Anti-mature BDNF antibody

STJ13100057 100 µl
EUR 512.4

Anti-mature BDNF antibody

STJ13100064 100 µl
EUR 512.4

Human BDNF ELISA Kit

E16HE0041 96T
EUR 833.33

Human BDNF ELISA Kit

EK5126 96 tests
EUR 449

Human BDNF ELISA Kit

E28L0224 96T
EUR 666.67

Human BDNF ELISA Kit

ELA-E0011h 96 Tests
EUR 988.8

Human BDNF ELISA Kit

EHB0036 96Tests
EUR 625.2

Human BDNF ELISA Kit

55R-1556 1 kit
EUR 615
Description: ELISA kit for detection of BDNF in the research laboratory

Human BDNF ELISA kit

LF-EK50005 1×96T
EUR 777.6

Human BDNF ELISA Kit

STJ150202 1 kit
EUR 494.4
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BDNF in human serum, plasma and other biological fluids

Human BDNF ELISA Kit

GWB-SKR197 96 Tests Ask for price

Human BDNF ELISA Kit

GWB-ZZD022 1x96 well plate Ask for price

BDNF (Human) ELISA Kit

EKA52013-5x96T 5x96T Ask for price

BDNF (Human) ELISA Kit

EKA52013-96T 96T
EUR 446.9

BDNF ELISA KIT|Human

EF006120 96 Tests
EUR 826.8

BDNF (human) ELISA Kit

K4788-100 each
EUR 1070.4

Human BDNF shRNA Plasmid

20-abx950425
  • Ask for price
  • Ask for price
  • 150 µg
  • 300 µg

Human BDNF Antibody (Biotin Conjugate)

32507-05121 150 ug
EUR 315

Mouse Monoclonal anti-Human BDNF Antibody

xAP-0037 100ug
EUR 280

Anti-Human BDNF Rabbit Polyclonal Antibody

TA328268 100 µg Ask for price

Human BDNF Recombinant Protein

100-005 10 µg
EUR 196.35
Description: BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residues and a propeptide of 110 amino acid residues. Recombinant Human BDNF is a 27.0 kDa homodimer of two 120 amino acid subunits linked by strong non-covalent interactions. Human and Mouse BDNF sequences are identical.

Human BDNF Recombinant Protein

100-005S 2 µg
EUR 92.4
Description: BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residues and a propeptide of 110 amino acid residues. Recombinant Human BDNF is a 27.0 kDa homodimer of two 120 amino acid subunits linked by strong non-covalent interactions. Human and Mouse BDNF sequences are identical.

Recombinant Human BDNF Protein

E40KMP1488 20ug
EUR 495

Recombinant Human BDNF Protein

RP00308 10 μg
EUR 265.2

Recombinant Human BDNF Protein

PROTP23560-3 10ug
EUR 380.4
Description: BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0 kDa homodimer of two 120 amino acid subunits linked by strong non-covalent interactions. Human and Mouse BDNF sequences are identical.

Human BDNF ELISA kit (4X96T)

LF-EK50006 4×96T
EUR 2641.2

Human BDNF knockout cell line

ABC-KH1413 1 vial Ask for price
Description: Human BDNF knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

BDNF/ Rat BDNF ELISA Kit

ELA-E0011r 96 Tests
EUR 1063.2

BDNF ORF Vector (Human) (pORF)

ORF000995 1.0 ug DNA
EUR 114

Human BDNF knockdown cell line

ABC-KD1413 1 vial Ask for price
Description: Human BDNF knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human BDNF Protein Lysate 20ug

IHUBDNFPLLY20UG each
EUR 213
Description: Human BDNF Protein Lysate 20ug

BDNF ELISA Kit (Human) (OKBB00127)

OKBB00127 96 Tests
EUR 606
Description: Description of target: BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system.1 BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically.2 BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system.3 BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state.4 It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury.5 The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13. 6.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 2 pg/ml

BDNF ELISA Kit (Human) (OKCD05621)

OKCD05621 96 Wells
EUR 746.4
Description: Description of target: BDNF is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression BDNF is reduced in both Alzheimer's and Huntington disease patients. BDNF may play a role in the regulation of stress response and in the biology of mood disorders.The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene, but the full-length nature of only some could be determined.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 12.1pg/mL

Human BDNF Signaling Primer Library

HBDNF-I 1 set
EUR 657.6

OPSA11059-10UG - Human BDNF Protein

OPSA11059-10UG 10ug
EUR 469

Nori® Human BDNF ELISA Kit

GR111085 96-well
EUR 461

BDNF antibody

10R-7738 500 ug
EUR 678
Description: Mouse monoclonal BDNF antibody

BDNF antibody

20R-1770 100 ug
EUR 807.6
Description: Rabbit polyclonal BDNF antibody

Combinatie van patch-clamp van hele cellen en kleurstoflading in acute hersenschijfjes met bulk-RNA-sequencing in embryonale tot verouderde muizen 

Single-cell electrophysiological recordings mixed with dye loading and immunohistochemistry present unparalleled single-cell decision of cell physiology, morphology, location, and protein expression. When correlated with bulk RNA sequencing, these knowledge can outline cell id and performance. Right here, we describe a protocol to organize acute mind slices from embryonic and postnatal mice for whole-cell patch clamp, dye loading and post-hoc immunohistochemistry, and cell isolation for bulk RNA sequencing. Whereas we concentrate on oligodendrocyte precursor cells, this protocol is relevant to different mind cells. For full particulars on the use and execution of this protocol, please consult with Spitzer et al. (2019).

Niet-lineaire normalisatie voor niet-UMI Single Cell RNA-Seq

Such noise outcomes from a mixed impact of unequal efficiencies within the capturing and counting of mRNA molecules, equivalent to extraction/amplification effectivity and sequencing depth. We present that such technical results will not be solely cell-specific, but additionally have an effect on genes in a different way, thus a easy cell-wise measurement issue adjustment will not be adequate. We current a non-linear normalization strategy that gives a cell- and gene-specific normalization issue for every gene in every cell. We present that the proposed normalization methodology (applied in “SC2P” package deal) reduces extra technical variation than competing strategies, with out decreasing organic variation. When technical results equivalent to sequencing depths will not be balanced between cell populations, SC2P normalization additionally removes the bias resulting from uneven technical noise. This methodology is relevant to scRNA-seq experiments that don’t use distinctive molecular identifier (UMI) thus retain amplification biases.

Functies en mechanismen van circulaire RNA’s bij het reguleren van stamceldifferentiatie 

Stem cells are a category of undifferentiated cells with nice self-renewal and differentiation capabilities that may differentiate into mature cells in particular tissue varieties. Stem cell differentiation performs important roles in physique homoeostasis, damage restore and tissue era. The vital capabilities of stem cell differentiation have resulted in quite a few research specializing in the complicated molecular mechanisms and numerous signalling pathways controlling stem cell differentiation.

Round RNAs (circRNAs) are a novel class of noncoding RNAs with a covalently closed construction current in eukaryotes. Quite a few research have highlighted vital organic capabilities of circRNAs, they usually play a number of regulatory roles in numerous physiological and pathological processes. Importantly, a number of strains of proof have proven the irregular expression of quite a few circRNAs throughout stem cell differentiation, and a few play a job in regulating stem cell differentiation, highlighting the function of circRNAs as novel biomarkers of stem cell differentiation and novel targets for stem cell-based remedy.

On this evaluate, we systematically summarize and talk about latest advances in our understanding of the roles and underlying mechanisms of circRNAs in modulating stem cell differentiation, thus offering steering for future research to research stem cell differentiation and stem cell-based remedy.

Abbreviations: CircRNAs: round RNAs; ESCs: embryonic stem cells; ADSCs: adipose-derived mesenchymal stem cells; ecircRNAs: exonic circRNAs; EIciRNAs: exon-intron circRNAs; eiRNAs: round intronic RNAs; tricRNAs: tRNA intronic circRNAs; pol II: polymerase II; snRNP: small nuclear ribonucleoprotein; m6A: N6-methyladenosine; AGO2: Argonaute 2; RBPs: RNA-binding proteins; MBNL: muscleblind-like protein 1;

MSCs: mesenchymal stem cells; hiPSCs: human induced pluripotent stem cells; hiPSC-CMs: hiPSC-derived cardiomyocytes; hBMSCs: human bone marrow mesenchymal stem cells; hADSCs: human adipose-derived mesenchymal stem cells; hDPSCs: human dental pulp stem cells; RNA-seq: high-throughput RNA sequencing; HSCs: haematopoietic stem cells; NSCs: neural stem cells; EpSCs: epidermal stem cells; hESCs: human embryonic stem cells; mESCs: murine embryonic stem cells; MNs: motor neurons; SSUP: small subunit processome; BMSCs: bone marrow-derived mesenchymal stem cells;

OGN: osteoglycin; GIOP: glucocorticoid‑induced osteoporosis; CDR1as: cerebellar degeneration-related protein 1 transcript; SONFH: steroid-induced osteogenesis of the femoral head; rBMSCs: rat bone marrow-derived mesenchymal stem cells; QUE: quercetin; AcvR1b: activin A receptor sort 1B; BSP: bone sialoprotein; mADSCs: mouse ADSCs; PTBP1: polypyrimidine tract-binding protein; ER: endoplasmic reticulum; hUCMSCs: MSCs derived from human umbilical wire; MSMSCs: maxillary sinus membrane stem cells;

SCAPs: stem cells from the apical papilla; MyoD: myogenic differentiation protein 1; MSTN: myostatin; MEF2C: myocyte enhancer issue 2C; BCLAF1: BCL2-associated transcription issue 1; EpSCs: epidermal stem cells; ISCs: intestinal stem cells; NSCs: neural stem cells; Lgr5+ ISCs: crypt base columnar cells; ILCs: innate lymphoid cells.

Circulair RNA hsa_circ_0000117 versnelt de proliferatie en invasie van maagkankercellen door de microRNA-337-3p / signaaltransducer en activator van transcriptie Three assen te reguleren 

Round RNA hsa_circ_0000117 is reportedly elevated in Gastric most cancers (GC), nonetheless, its function is unexplored. Hsa_circ_0000117 expression and performance in GC was investigated utilizing normal cell phenotypic and expression assays. Pull-down and luciferase reporter assays additionally elucidated hsa_circ_0000117 mechanisms. Within the current examine, we noticed elevated hsa_circ_0000117 and sign transducer and activator of transcription 3 (STAT3) expression, whereas microRNA-337-3p (miR-337-3p) was decreased in GC cells.

Depleted hsa_circ_0000117 decreased GC proliferation and invasion. Hsa_circ_0000117 was additionally recognized as a miR-337-3p sponge. Additionally, STAT3 was recognized as a miR-337-3p goal. Equally, rescue assays indicated STAT3 overexpression (or miR-337-3p inhibition) reversed hsa_circ_0000117 results in GC development.

Thus, our knowledge steered hsa_circ_0000117 exhibited oncogene properties together with the hsa_circ_0000117/miR-337-3p/STAT3 axis in GC, probably offering a brand new therapeutic goal for GC.Abbreviations GC: gastric most cancers; STAT3: Sign transducer and activator of transcription 3; circRNA: Round RNA; miRNA: microRNA; DMEM: Dulbecco’s modified Eagle’s medium; FBS: fetal bovine serum;

PVDF: polyvinylidene fluoride; CCK-8: Cell counting kit-8; qRT-PCR: Quantitative real-time PCR; SDS-PAGE: sodium dodecyl sulfate polyacrylamide gel electrophoresis; TNM: TNM Classification of Malignant Tumors; mTOR: mechanistic goal of rapamycin; ANOVA: one-way evaluation of variance.

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