Koud-induceerbaar RNA-bindend eiwit (CIRP) versterkt

Koud-induceerbaar RNA-bindend eiwit (CIRP) versterkt de door urinezuur geïnduceerde IL-1β-productie 

Background: Gout is an autoinflammatory illness pushed by interleukin-1 (IL-1) induction in response to uric acid crystals. IL-1β manufacturing is depending on inflammasome activation, which requires a priming sign, adopted by an activating sign. The cold-inducible RNA-binding protein (CIRP) has been just lately recognized as a damage-associated molecular sample (DAMP). On this research, we evaluated the roles of CIRP in monosodium urate (MSU)-mediated IL-1β secretion utilizing human neutrophils.

Strategies: Human neutrophils have been stimulated by MSU within the presence or absence of CIRP priming to find out NLRP3 inflammasome activation and subsequent caspase-1 activation and IL-1β manufacturing. Mobile supernatants have been analyzed by enzyme-linked immunosorbent assay (ELISA) to find out the presence of IL-1β or caspase-1 (p20). The mobile supernatants and lysates have been additionally analyzed by immunoblotting utilizing anti-cleaved IL-1β or anti-cleaved caspase-1 antibodies.

Outcomes: Neither CIRP nor MSU stimulation alone induced ample IL-1β secretion from neutrophils. Nonetheless, MSU stimulation induced IL-1β secretion from CIRP-primed neutrophils in a dose-dependent method. This MSU-induced IL-1β secretion from CIRP-primed neutrophils was accompanied by the induction of cleaved IL-1β (p17), which was inhibited by the pretreatment of MCC950, a particular inhibitor for NLRP3. Moreover, cleaved caspase-1 was induced within the mobile lysates of CIRP/MSU-treated neutrophils. Moreover, CIRP stimulation induced the protein expression of pro-IL-1β in neutrophils.

Conclusions: Our knowledge point out that CIRP, an endogenous stress molecule, triggers uric acid-induced mature IL-1β induction as a priming stimulus for NLRP3 inflammasome in human neutrophils. We suggest that CIRP acts as an vital proinflammatory stimulant that primes and prompts inflammasome and pro-IL-1β processing in response to uric acid in innate immune cells.

blsystems
blsystems

Anti-Angiogenin antibody

PAab00390 100 ug
EUR 355

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Anti-Angiogenin/ANG Antibody

A00146 100ug/vial
EUR 294

Mouse Anti Human Angiogenin Monoclonal Antibody

CABT-49293MH 0.15 mg
EUR 1053

Angiogenin

PR27204 5 ug
EUR 191

Human Angiogenin (ANG)

1-CSB-RP057294h
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Angiogenin(ANG),partial expressed in E.coli

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Angiogenin Protein

20-abx261289
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Angiogenin antibody

70R-15437 100 ug
EUR 327
Description: Rabbit polyclonal Angiogenin antibody

Angiogenin Human Recombinant Protein

PROTP03950 Regular: 20ug
EUR 317
Description: ANG Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 144 amino acids (25-147) and having a molecular mass of 16.4 kDa.;ANG is fused to a 21 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Human Angiogenin (ANG) Protein

20-abx166508
  • EUR 648.00
  • EUR 272.00
  • EUR 1943.00
  • EUR 759.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Angiogenin (ANG) Antibody

31388-05111 150 ug
EUR 261

Human Angiogenin ELISA kit

E01A0501-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Human Angiogenin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Angiogenin ELISA kit

E01A0501-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Human Angiogenin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Angiogenin ELISA kit

E01A0501-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Human Angiogenin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Angiogenin ELISA kit

55R-2081 96 tests
EUR 712
Description: ELISA Kit for detection of Angiogenin in the research laboratory

Angiogenin (108-122)

HY-P1516 10mg
EUR 980

Angiogenin (ANG) Antibody

20-abx132357
  • EUR 328.00
  • EUR 829.00
  • EUR 425.00
  • EUR 154.00
  • EUR 258.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Angiogenin (ANG) Antibody

20-abx128903
  • EUR 314.00
  • EUR 133.00
  • EUR 829.00
  • EUR 439.00
  • EUR 272.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Angiogenin (ANG) Antibody

20-abx129495
  • EUR 411.00
  • EUR 133.00
  • EUR 1135.00
  • EUR 551.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Angiogenin (ANG) Antibody

20-abx121013
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Angiogenin (ANG) Antibody

20-abx110971
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Angiogenin (ANG) Antibody

20-abx109530
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Angiogenin (ANG) Antibody

20-abx100298
  • EUR 411.00
  • EUR 133.00
  • EUR 1135.00
  • EUR 551.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Angiogenin (ANG) Antibody

20-abx100299
  • EUR 425.00
  • EUR 133.00
  • EUR 1177.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Angiogenin (ANG) Antibody

20-abx100300
  • EUR 453.00
  • EUR 133.00
  • EUR 1288.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Angiogenin (ANG) Antibody

20-abx100301
  • EUR 453.00
  • EUR 133.00
  • EUR 1288.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Angiogenin (ANG) Antibody

20-abx100302
  • EUR 356.00
  • EUR 913.00
  • EUR 467.00
  • EUR 154.00
  • EUR 272.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Angiogenin (ANG) Antibody

20-abx001302
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Angiogenin (108-122)

5-00673 4 x 1mg Ask for price

Angiogenin (108-123)

5-00674 4 x 1mg Ask for price

Angiogenin (ANG) Antibody

20-abx171213
  • EUR 913.00
  • EUR 467.00
  • 1 mg
  • 200 ug

Angiogenin (ANG) Antibody

20-abx323491
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

Angiogenin (ANG) Antibody

abx230390-100ug 100 ug
EUR 481

Angiogenin (ANG) Antibody

20-abx339590
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Angiogenin (ANG) Antibody

abx148162-100ug 100 ug
EUR 439

Angiogenin (ANG) Antibody

20-abx141176
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul

Angiogenin (ANG) Antibody

20-abx175378
  • EUR 1316.00
  • EUR 634.00
  • 1 mg
  • 200 ug

Angiogenin Polyclonal Antibody

42438-100ul 100ul
EUR 333

Recombinant Angiogenin (ANG)

4-RPA007Bo01
  • EUR 504.99
  • EUR 238.00
  • EUR 1618.72
  • EUR 606.24
  • EUR 1112.48
  • EUR 401.00
  • EUR 3896.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Bovine Angiogenin expressed in: E.coli

Recombinant Angiogenin (ANG)

4-RPA007Hu01
  • EUR 458.40
  • EUR 226.00
  • EUR 1444.00
  • EUR 548.00
  • EUR 996.00
  • EUR 370.00
  • EUR 3460.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Angiogenin expressed in: E.coli

Recombinant Angiogenin (ANG)

4-RPA007Mu01
  • EUR 478.11
  • EUR 231.00
  • EUR 1517.92
  • EUR 572.64
  • EUR 1045.28
  • EUR 383.00
  • EUR 3644.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Angiogenin expressed in: E.coli

Recombinant Angiogenin (ANG)

4-RPA007Mu02
  • EUR 478.11
  • EUR 231.00
  • EUR 1517.92
  • EUR 572.64
  • EUR 1045.28
  • EUR 383.00
  • EUR 3644.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Angiogenin expressed in: E.coli

Recombinant Angiogenin (ANG)

4-RPA007Po01
  • EUR 530.08
  • EUR 245.00
  • EUR 1712.80
  • EUR 637.60
  • EUR 1175.20
  • EUR 418.00
  • EUR 4132.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pig Angiogenin expressed in: E.coli

Recombinant Angiogenin (ANG)

4-RPA007Ra01
  • EUR 485.28
  • EUR 233.00
  • EUR 1544.80
  • EUR 581.60
  • EUR 1063.20
  • EUR 388.00
  • EUR 3712.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Angiogenin expressed in: E.coli

Angiogenin (108-123)

H-8020.0001 1.0mg
EUR 441
Description: Sum Formula: C83H132N26O24; CAS# [112173-48-5]

Angiogenin (108-123)

H-8020.0005 5.0mg
EUR 1675
Description: Sum Formula: C83H132N26O24; CAS# [112173-48-5]

Angiogenin (108-122)

H-8095.0001 1.0mg
EUR 212
Description: Sum Formula: C78H125N25O23; CAS# [112173-49-6]

Angiogenin (108-122)

H-8095.0005 5.0mg
EUR 756
Description: Sum Formula: C78H125N25O23; CAS# [112173-49-6]

Angiogenin antibody (HRP)

60R-2121 100 ug
EUR 327
Description: Rabbit polyclonal Angiogenin antibody (HRP)

Angiogenin antibody (FITC)

60R-2122 100 ug
EUR 327
Description: Rabbit polyclonal Angiogenin antibody (FITC)

Angiogenin antibody (biotin)

60R-2123 100 ug
EUR 349
Description: Rabbit polyclonal Angiogenin antibody (biotin)

Angiogenin Recombinant Protein

91-183 0.05 mg
EUR 464
Description: Angiogenin belongs to the pancreatic ribonuclease family. Angiogenin is primarily expressed in the liver. It may act as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Angiogenin is a potent stimulator of new blood vessel formation. And Angiogenin is endocytosed and translocated to the nucleus by binding to actin on the surface of endothelial cells. Angiogenic activity is regulated by interaction with RNH1 in vivo. In addition, Angiogenin is associated with susceptibility to amyotrophic lateral sclerosis, which is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord.

Human Angiogenin(ANG)ELISA Kit

GA-E1240HM-48T 48T
EUR 289

Human Angiogenin(ANG)ELISA Kit

GA-E1240HM-96T 96T
EUR 466

Human ANG/ Angiogenin ELISA Kit

E0142Hu 1 Kit
EUR 537

Human Angiogenin,ANG ELISA Kit

201-12-1224 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Angiogenin (ANG) CLIA Kit

20-abx490752
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Angiogenin (ANG) ELISA Kit

abx350014-96tests 96 tests
EUR 668

Human Angiogenin (ANG) ELISA Kit

20-abx150645
  • EUR 5640.00
  • EUR 3009.00
  • EUR 707.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Angiogenin (ANG) CLIA Kit

abx195094-96tests 96 tests
EUR 825

Human Angiogenin (ANG) ELISA Kit

RDR-RNASE5-Hu-48Tests 48 Tests
EUR 383

Human Angiogenin (ANG) ELISA Kit

RDR-RNASE5-Hu-96Tests 96 Tests
EUR 525

Human Angiogenin (ANG) ELISA Kit

RD-RNASE5-Hu-48Tests 48 Tests
EUR 367

Human Angiogenin (ANG) ELISA Kit

RD-RNASE5-Hu-96Tests 96 Tests
EUR 502

Human Angiogenin ELISA Kit (ANG)

RK00879 96 Tests
EUR 521

ELISA kit for Human Angiogenin

EK1015 96 tests
EUR 452
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Angiogenin in samples from serum, plasma, tissue homogenates and other biological fluids.

Human ANG(Angiogenin) ELISA Kit

EH0034 96T
EUR 476.25
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 46.875pg/ml

Human Angiogenin (ANG) ELISA Kit

DLR-RNASE5-Hu-48T 48T
EUR 380
Description: A sandwich quantitative ELISA assay kit for detection of Human Angiogenin (ANG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Angiogenin (ANG) ELISA Kit

DLR-RNASE5-Hu-96T 96T
EUR 485
Description: A sandwich quantitative ELISA assay kit for detection of Human Angiogenin (ANG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Angiogenin (ANG) Polyclonal Antibody (Human)

4-PAA007Hu01
  • EUR 194.00
  • EUR 1678.00
  • EUR 436.00
  • EUR 234.00
  • EUR 191.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Angiogenin (ANG)

Human Angiogenin, ANG ELISA Kit

CSB-E04498h-24T 1 plate of 24 wells
EUR 165
Description: Quantitative sandwich ELISA kit for measuring Human Angiogenin, ANG in samples from serum, plasma, cell culture supernates, urine, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Angiogenin, ANG ELISA Kit

1-CSB-E04498h
  • EUR 511.00
  • EUR 3491.00
  • EUR 1866.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitative sandwich ELISA kit for measuring Human Angiogenin, ANG in samples from serum, plasma, cell culture supernates, urine, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Angiogenin (ANG) Monoclonal Antibody (Human)

4-MAA007Hu22
  • EUR 249.00
  • EUR 2549.00
  • EUR 634.00
  • EUR 313.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Angiogenin (ANG)

Human Angiogenin (ANG) ELISA Kit

SEA007Hu-10x96wellstestplate 10x96-wells test plate
EUR 3409.41
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiogenin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Angiogenin (ANG) ELISA Kit

SEA007Hu-1x48wellstestplate 1x48-wells test plate
EUR 368.42
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiogenin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Angiogenin (ANG) ELISA Kit

SEA007Hu-1x96wellstestplate 1x96-wells test plate
EUR 483.46
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiogenin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Angiogenin (ANG) ELISA Kit

SEA007Hu-5x96wellstestplate 5x96-wells test plate
EUR 1875.57
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiogenin (ANG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Angiogenin (ANG) ELISA Kit

4-SEA007Hu
  • EUR 3460.00
  • EUR 1826.00
  • EUR 484.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Angiogenin (ANG) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Angiogenin(ANG)ELISA Kit

QY-E00700 96T
EUR 361

Recombinant Human Angiogenin/ANG/RNASE5

C203-10ug 10ug
EUR 131
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Recombinant Human Angiogenin/ANG/RNASE5

C203-1mg 1mg
EUR 2283
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Recombinant Human Angiogenin/ANG/RNASE5

C203-500ug 500ug
EUR 1613
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Recombinant Human Angiogenin/ANG/RNASE5

C203-50ug 50ug
EUR 273
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Human ANG / Angiogenin ELISA kit

LF-EK50621 1×96T
EUR 648

Human Angiogenin,ANG ELISA Kit

CN-03157H1 96T
EUR 378

Human Angiogenin,ANG ELISA Kit

CN-03157H2 48T
EUR 289

Goat Anti-Human Angiogenin protein IgG #1, IgG aff pure

ANGN12-A 100 ug
EUR 482

ELISA kit for Human ANG (Angiogenin)

ELK1830 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Angiogenin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Human ANG (Angiogenin)

E-EL-H0006 1 plate of 96 wells
EUR 377
Description: A sandwich ELISA kit for quantitative measurement of Human ANG (Angiogenin) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human ANG (Angiogenin)

ELK1004 1 plate of 96 wells
EUR 372
Description: A sandwich ELISA kit for detection of Angiogenin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

CLIA kit for Human ANG (Angiogenin)

E-CL-H0006 1 plate of 96 wells
EUR 584
Description: A sandwich CLIA kit for quantitative measurement of Human ANG (Angiogenin) in samples from Serum, Plasma, Cell supernatant

Human Angiogenin (ANG) Antibody (Biotin Conjugate)

31388-05121 150 ug
EUR 369

Angiogenin/ANG ELISA Kit (Human) (OKBB00126)

OKBB00126 96 Tests
EUR 544
Description: Description of target: Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. The human angiogenin gene was mapped to chromosome 14q11.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 12 pg/ml

ELISA kit for Human Angiogenin,ANG

EK0135 96 tests
EUR 612
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Angiogenin,ANG in samples from serum, plasma, tissue homogenates and other biological fluids.

Angiogenin (ANG) Polyclonal Antibody (Human), APC

4-PAA007Hu01-APC
  • EUR 266.00
  • EUR 2141.00
  • EUR 629.00
  • EUR 326.00
  • EUR 185.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Angiogenin (ANG). This antibody is labeled with APC.

Genoombrede identificatie van sucrose-niet-fermenterende-1-gerelateerde proteïnekinase (SnRK) -genen in gerst- en RNA-seq-analyses van hun expressie als reactie op behandeling met abscisinezuur 

Background: Sucrose nonfermenting-1 (SNF1)-related protein kinases (SnRKs) play vital roles in regulating metabolism and stress responses in vegetation, offering a conduit for crosstalk between metabolic and stress signalling, in some circumstances involving the stress hormone, abscisic acid (ABA).

The burgeoning and divergence of the plant gene household has led to the evolution of three subfamilies, SnRK1, SnRK2 and SnRK3, of which SnRK2 and SnRK3 are distinctive to vegetation. Due to this fact, the research of SnRKs in crops could result in the event of methods for breeding crop varieties which can be extra resilient below stress situations. Within the current research, we describe the SnRK gene household of barley (Hordeum vulgare), the widespread cultivation of which will be attributed to its good adaptation to completely different environments.

Outcomes: The barley HvSnRK gene household was elucidated in its entirety from publicly-available genome knowledge and located to comprise 50 genes. Phylogenetic analyses assigned six of the genes to the HvSnRK1 subfamily, 10 to HvSnRK2 and 34 to HvSnRK3. The search was validated by making use of it to Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa) genome knowledge, figuring out 50 SnRK genes in rice (4 OsSnRK1, 11 OsSnRK2 and 35 OsSnRK3) and 39 in Arabidopsis (three AtSnRK1, 10 AtSnRK2 and 26 AtSnRK3).

Particular motifs have been recognized within the encoded barley proteins, and a number of putative regulatory parts have been discovered within the gene promoters, with light-regulated parts (LRE), ABA response parts (ABRE) and methyl jasmonate response parts (MeJa) the most typical. RNA-seq evaluation confirmed that lots of the HvSnRK genes responded to ABA, some positively, some negatively and a few with complicated time-dependent responses.

Conclusions: The barley HvSnRK gene household is massive, comprising 50 members, subdivided into HvSnRK1 (6 members), HvSnRK2 (10 members) and HvSnRK3 (34 members), displaying differential constructive and unfavourable responses to ABA.

Protomeeruitlijning moduleert de specificiteit van RNA-substraatherkenning door Ire1 

The unfolded protein response (UPR) maintains protein folding homeostasis within the endoplasmic reticulum (ER). In metazoan cells, the Ire1 department of the UPR initiates two useful outputs-non-conventional mRNA splicing and selective mRNA decay (RIDD). In contrast, Ire1 orthologs from Saccharomyces cerevisiae and Schizosaccharomyces pombe are specialised for less than splicing or RIDD, respectively. Beforehand, we confirmed that the useful specialization lies in Ire1’s RNase exercise, which is both stringently splice-site particular or promiscuous (W. Li et al., 2018). Right here, we developed an assay that experiences on Ire1’s RNase promiscuity.

We discovered that conversion of two amino acids throughout the RNase area of S. cerevisiae Ire1 to their S. pombe counterparts rendered it promiscuous. Utilizing biochemical assays and computational modeling, we present that the mutations rewired a pair of salt bridges at Ire1 RNase area’s dimer interface, altering its protomer alignment. Thus, Ire1 protomer alignment impacts its substrates specificity.

Paired-end small RNA-sequencing onthult een mogelijke overschatting in het isomiR-sequentierepertoire dat eerder werd gerapporteerd op foundation van conventionele single-read data-analyse

Background: Subsequent era sequencing has allowed the discovery of miRNA isoforms, termed isomiRs. Some isomiRs are derived from imprecise processing of pre-miRNA precursors, resulting in size variants. Further variability is launched by non-templated addition of bases on the ends or enhancing of inner bases, leading to base variations relative to the template DNA sequence. We hypothesized that some part of the isomiR variation reported thus far may very well be attributable to systematic technical noise and never actual.

Outcomes: Now we have developed the XICRA pipeline to investigate small RNA sequencing knowledge on the isomiR degree. We exploited its potential to make use of single or merged reads to check isomiR outcomes derived from paired-end (PE) reads with these from single reads (SR) to deal with whether or not detectable sequence variations relative to canonical miRNAs present in isomiRs are true organic variations or the results of errors in sequencing. Now we have detected non-negligible systematic variations between SR and PE knowledge which primarily have an effect on putative internally edited isomiRs, and at a a lot smaller frequency terminal size altering isomiRs. That is related for the identification of true isomiRs in small RNA sequencing datasets.

Conclusions: We conclude that potential artifacts derived from sequencing errors and/or knowledge processing might end in an overestimation of abundance and variety of miRNA isoforms. Efforts in annotating the isomiRnome ought to take this under consideration.

Chromatine-tracering en multiplex-beeldvorming van nucleoomarchitecturen (MINA) en RNA’s in afzonderlijke zoogdiercellen en -weefsel 

  • The genome is hierarchically organized into a number of 3D architectures, together with chromatin loops, domains, compartments and areas related to nuclear lamina and nucleoli. Modifications in these architectures have been related to regular growth, growing old and a variety of illnesses.
  • Regardless of its crucial significance, understanding how the genome is spatially organized in single cells, how group varies in several cell varieties in mammalian tissue and the way group impacts gene expression stays a significant problem. Earlier approaches have been restricted by a scarcity of capability to immediately hint chromatin folding in 3D and to concurrently measure genomic group in relation to different nuclear parts and gene expression in the identical single cells.
  • Now we have developed an image-based 3D genomics method termed ‘chromatin tracing’, which permits direct 3D tracing of chromatin folding alongside particular person chromosomes in single cells. Extra just lately, we additionally developed multiplexed imaging of nucleome architectures (MINA), which permits simultaneous measurements of multiscale chromatin folding, associations of genomic areas with nuclear lamina and nucleoli and replica numbers of quite a few RNA species in the identical single cells in mammalian tissue.
  • Right here, we offer detailed protocols for chromatin tracing in cell traces and MINA in mammalian tissue, which take 3-Four d for experimental work and 2-Three d for knowledge evaluation. We anticipate these developments to be broadly relevant and to have an effect on many traces of analysis on 3D genomics by depicting multiscale genomic architectures related to gene expression, in various kinds of cells and tissue present process completely different organic processes.

Polyclonal PROX-1-S514 Antibody

APR12985G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PROX-1-S514 . This antibody is tested and proven to work in the following applications:

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Anti-Apaf-1 (human) Monoclonal Antibody (2E12)

M00889-1 100ug
EUR 432
Description: Rat Monoclonal Apaf-1 (human) Antibody (2E12). Validated in ELISA, IP, IF, WB and tested in Human.

Anti-PARP-1 Antibody

A00122-1 100ul
EUR 397
Description: Rabbit Polyclonal PARP-1 Antibody. Validated in ELISA, IP, IF, WB and tested in Human, Mouse.

Anti-Presenilin 1 Antibody

A00138-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Presenilin 1 Antibody (PSEN1) detection. Tested with WB, IHC in Human, Mouse, Rat.

Anti-PAI-1 Antibody

A00637-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for PAI-1 Antibody (SERPINE1) detection.tested for WB in Human, Mouse, Rat.

Anti-Flk-1 Antibody

A00901-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Flk-1 Antibody (KDR) detection.tested for WB in Human, Mouse.

Anti-EDG-1 Antibody

A01502-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for EDG-1 Antibody (S1PR1) detection.tested for WB in Human, Mouse, Rat.

Anti-ROBO-1 Antibody

A01530-1 100ug
EUR 455
Description: Rabbit Polyclonal ROBO-1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-TFIIIB90-1 Antibody

A03761-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for TFIIIB90-1 Antibody (BRF1) detection.tested for WB in Human, Mouse.

Anti-Atrophin-1 Antibody

A03828-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Atrophin-1 Antibody (ATN1) detection. Tested with WB in Human, Mouse, Rat.

Anti-CNG-1 Antibody

A05494-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for CNG-1 Antibody (CNGA1) detection. Tested with WB in Human, Mouse, Rat.

Anti-Periphilin 1 Antibody

A06996-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Periphilin 1 Antibody (PPHLN1) detection.tested for WB in Human, Mouse.

Anti-Lyl-1 Antibody

A07491-1 100ul
EUR 397
Description: Rabbit Polyclonal Lyl-1 Antibody. Validated in IHC and tested in Human, Mouse, Rat.

Anti-GLI-1 Antibody

A07972-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for GLI-1 Antibody (ACSS1) detection. Tested with WB in Human, Mouse, Rat.

Anti-Cerebellin 1 Antibody

A09176-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Cerebellin 1 Antibody (CBLN1) detection. Tested with WB in Human, Mouse, Rat.

Anti-DOC-1 Antibody

A09467-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for DOC-1 Antibody (CDK2AP1) detection. Tested with WB in Human, Mouse.

Anti-Bag-1 Antibody

A02423-1 50 ul
EUR 397
Description: Rabbit Polyclonal Bag-1 Antibody. Validated in IP, IHC and tested in Bovine, Canine, Human, Mouse, Rat.

Anti-TUB 1 Antibody

A02917-1 100ug/vial
EUR 294

Anti-Dok-1 Antibody

A03039-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Dok-1 Antibody (DOK1) detection.tested for WB in Human, Mouse, Rat.

Anti-NPDC-1 Antibody

A12846-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for NPDC-1 Antibody (NPDC1) detection. Tested with WB in Human, Mouse, Rat.

Anti-P-glycoprotein (human) Monoclonal Antibody (JSB-1)

M00049-1 125ug
EUR 586
Description: Mouse Monoclonal P-glycoprotein (human) Antibody (JSB-1). Validated in IF and tested in Human.

Anti-TTF-1

DB-089-1 1 ml
EUR 750
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Esrp-1 Monoclonal Antibody

M06068-1 100uL
EUR 443
Description: Mouse Monoclonal Esrp-1 Antibody. Validated in WB and tested in Human.

Anti-Presenilin 1 Monoclonal Antibody

M00138-1 100ug
EUR 397
Description: Rabbit Monoclonal Presenilin 1 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-Galectin 1 Monoclonal Antibody

M00470-1 100ug
EUR 397
Description: Rabbit Monoclonal Galectin 1 Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat.

Anti-DJ-1 Monoclonal Antibody

M00757-1 100ul
EUR 397
Description: Mouse Monoclonal DJ-1 Antibody. Validated in IHC, WB and tested in Bovine, Human.

Anti-Enolase 1 Monoclonal Antibody

M01250-1 100ul
EUR 397
Description: Anti-Enolase 1 Monoclonal Antibody tested in WB, IF, ICC, IHC, reactive to Human, rat, mouse, cow, pig, horse

Anti-Dynamin 1 Monoclonal Antibody

M02536-1 100ug
EUR 397
Description: Rabbit Monoclonal Dynamin 1 Antibody. Validated in IF, WB and tested in Human, Mouse, Rat.

Anti-Angiopoietin 1/ANGPT1 Antibody

PA1333-1 100ug/vial
EUR 334

Anti-Galectin 1/LGALS1 Antibody

PB9240-1 100ug/vial
EUR 334

Anti-TNF Receptor 1 Antibody

A00294-1 200ug
EUR 522
Description: Rabbit Polyclonal TNF Receptor 1 Antibody. Validated in IP, IHC, WB and tested in Human, Mouse, Rat.

Anti-Galectin 1/Lgals1 Antibody

A00470-1 100ug/vial
EUR 334

Anti-LOX-1/OLR1 Antibody

A00760-1 100ug/vial
EUR 294

Anti-Syndecan-1/SDC1 Antibody

A00991-1 100ug/vial
EUR 294

Anti-IRAK-1/IRAK1 Antibody

A01021-1 100ug/vial
EUR 334

Anti-Neurexin 1/NRXN1 Antibody

A01490-1 100ug/vial
EUR 334

Anti-Hexokinase 1/HK1 Antibody

A01504-1 100ug/vial
EUR 334

Anti-Musashi 1/Msi1 Antibody

A05052-1 100ug/vial
EUR 334

Anti-Pyrophosphatase 1/PPA1 Antibody

A07485-1 100ug/vial
EUR 334

Anti-TCP-1 eta Antibody

A08169-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for TCP-1 eta Antibody (CCT7) detection. Tested with WB in Human, Mouse, Rat.

Anti-Relaxin 1/RLN1 Antibody

A08367-1 100ug/vial
EUR 334

Anti-TCP-1 zeta Antibody

A09373-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for TCP-1 zeta Antibody (CCT6A) detection.tested for WB in Human, Mouse, Rat.

Anti-Synaptotagmin 1/SYT1 Antibody

A02314-1 100ug/vial
EUR 334

Anti-Syntenin-1 Monoclonal Antibody

A02475-1 100ul
EUR 397
Description: Mouse Monoclonal Antibody for Syntenin-1 Antibody (SDCBP) detection. Tested with WB in Human, Rat, Dog, Pig.

Anti-Dsg1/Desmoglein 1 Antibody

A02655-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Dsg1 Antibody (DSG1) detection.tested for IHC, WB in Human, Mouse, Rat.

Anti-CAF-1 p150 Antibody

A02732-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for CAF-1 p150 Antibody (CHAF1A) detection. Tested with WB in Human, Mouse.

Anti-Talin 1/TLN1 Antibody

A02859-1 100ug/vial
EUR 334

Anti-Islet 1/ISL1 Antibody

A02969-1 100ug/vial
EUR 334

Anti-CHST8/Galnac4St 1 Antibody

A10989-1 100ul
EUR 397
Description: Rabbit Polyclonal CHST8/Galnac4St 1 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-HLA-G (human) Monoclonal Antibody (MEM-G/1)

M01235-1 100ug
EUR 397
Description: Mouse Monoclonal HLA-G (human) Antibody (MEM-G/1). Validated in IHC, WB and tested in Human.

Anti-HO-1 Monoclonal Antibody (HO-1-2)

M00253-1 1mg
EUR 397
Description: Mouse Monoclonal HO-1 Antibody (HO-1-2). Validated in Flow Cytometry, IHC, WB and tested in Human, Mouse, Rat.

Anti-Sumo 1 Rabbit Monoclonal Antibody

M00631-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Sumo 1 Antibody. Validated in IP, IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-Cytokeratin 1 Rabbit Monoclonal Antibody

M01639-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Cytokeratin 1 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-Mitofusin 1 Antibody (monoclonal, 3H3)

M02172-1 100ug/vial
EUR 294

Anti-Phospho-Beclin-1 (Ser295) Antibody

P00327-1 100ul
EUR 398
Description: Rabbit Polyclonal Phospho-Beclin-1 (Ser295) Antibody. Validated in WB and tested in Human.

Anti-Phospho-Raf-1 (Ser642) Antibody

P00446-1 100ul
EUR 398
Description: Rabbit Polyclonal Phospho-Raf-1 (Ser642) Antibody. Validated in WB and tested in Human, Rat.

Anti-Integrin alpha 1/ITGA1 Antibody

PA1045-1 100ug/vial
EUR 334

Anti-Caspase-1(P20)/CASP1 Antibody

PA1440-1 100ug/vial
EUR 294

Anti-VEGF Receptor 1/FLT1 Antibody

PA1966-1 100ug/vial
EUR 294

Anti-HIF-1 alpha/HIF1A Antibody

A00013-1 100ug/vial
EUR 294

Anti-Cleaved-Notch 1 (V1754) Antibody

A00033-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Cleaved-Notch 1 (V1754) Antibody (NOTCH1) detection.tested for WB in Human, Mouse, Rat.

Anti-HDAC-1 (C-terminus) Antibody

A00256-1 100uL
EUR 443
Description: Rabbit Polyclonal HDAC-1 (C-terminus) Antibody. Validated in IF, IHC, WB and tested in Human.

Anti-VEGF Receptor 1/FLT1 Antibody

A00534-1 100ug/vial
EUR 294

Anti-Liver Carboxylesterase 1/CES1 Antibody

A01741-1 100ug/vial
EUR 334

Anti-Hyaluronan synthase 1/HAS1 Antibody

A04784-1 100ug/vial
EUR 334

Anti-CELSR3/Flamingo Homolog 1 Antibody

A07204-1 100ul
EUR 397
Description: Rabbit Polyclonal CELSR3/Flamingo Homolog 1 Antibody. Validated in IF and tested in Human, Mouse, Rat.

Anti-LPCAT2/Acyltransferase Like 1 Antibody

A07471-1 100ul
EUR 397
Description: Rabbit Polyclonal LPCAT2/Acyltransferase Like 1 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-EBP50/NHERF-1/SLC9A3R1 Antibody

A02427-1 100ug/vial
EUR 334

Anti-IL1R2/Il 1 Rii Antibody

A03106-1 1 ml
EUR 397
Description: Rabbit Polyclonal IL1R2/Il 1 Rii Antibody. Validated in IP, WB and tested in Human.

Anti-EBV/LMP-1

DB-060-1 1 ml
EUR 750
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-EBV/LMP-1

DB-061-1 1 ml
EUR 750
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-CCR4 (human) Antibody

A00755-1 200ug
EUR 397
Description: Goat Polyclonal CCR4 (human) Antibody. Validated in IF, IHC and tested in Human.

Anti-BOB-1/OBF-1 Rabbit Monoclonal Antibody, Clone#RM378

M04431-1 100uL
EUR 385
Description: Anti-BOB-1/OBF-1 Rabbit Monoclonal Antibody, Clone#RM378 tested in WB, IHC, reactive to Human

AXMIR-1 RNA oligo anti-miRNA-1-3p with Xmotif

AXMIR-1 10 reactions
EUR 438

Anti-Musashi 1/Msi1 Antibody (monoclonal, 2B9)

M05052-1 100ug/vial
EUR 334

Anti-Visinin-like Protein 1 Monoclonal Antibody

M06959-1 100ul
EUR 397
Description: Mouse Monoclonal Visinin-like Protein 1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-HMGB1/Hmg 1 Rabbit Monoclonal Antibody

M00066-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal HMGB1/Hmg 1 Antibody. Validated in Flow Cytometry, IF, IHC, ICC, WB and tested in Human, Mouse, Rat.

Anti-p27 KIP 1 Rabbit Monoclonal Antibody

M00173-1 100ug/vial
EUR 397
Description: Anti-p27 KIP 1 Rabbit Monoclonal Antibody tested for Flow Cytometry, IP, IF, IHC, ICC, WB in Human, Rat

Anti-Caveolin-1/CAV1 Antibody (monoclonal, 12C7)

M00179-1 100ug/vial
EUR 334

Anti-MHC class 1 Rabbit Monoclonal Antibody

M00194-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal MHC class 1 Antibody. Validated in IP, IF, IHC, WB and tested in Human.

Anti-Integrin beta 1 Rabbit Monoclonal Antibody

M00772-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Integrin beta 1 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Anti-ARG1/Arginase 1 Rabbit Monoclonal Antibody

M01106-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal ARG1/Arginase 1 Antibody. Validated in IP, WB and tested in Human, Mouse, Rat.

Anti-MLANA/Mart 1 Rabbit Monoclonal Antibody

M02033-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal MLANA/Mart 1 Antibody. Validated in IF, WB and tested in Human.

Anti-TCP-1α Monoclonal Antibody (91a)

M02389-1 200ug
EUR 498
Description: Rat Monoclonal TCP-1α Antibody (91a). Validated in Flow Cytometry, IP, IF, WB and tested in Human, Mouse, Rat.

Anti-DSG1/Desmoglein 1 Rabbit Monoclonal Antibody

M02655-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal DSG1/Desmoglein 1 Antibody. Validated in IF, IHC, ICC, WB and tested in Human, Mouse, Rat.

Anti-Phospho-Glycogen Synthase 1 (S645) Antibody

P03512-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Phospho-Glycogen Synthase 1 (S645) Antibody (GYS1) detection.tested for WB in Human, Mouse, Rat.

Anti-Angiotensin Converting Enzyme 1/ACE Antibody

PA2196-1 100ug/vial
EUR 334

Anti-GABA B Receptor 1/GABBR1 Antibody

A07297-1 100ug/vial
EUR 294

Anti-Vangl1/Vang Like Protein 1 Antibody

A07587-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Vangl1 Antibody (VANGL1) detection. Tested with WB in Human, Mouse.

Anti-E74 like factor 1/ELF1 Antibody

A03187-1 100ug/vial
EUR 294

miRZip-1 anti-miR-1 microRNA construct

MZIP1-PA-1 Bacterial Streak
EUR 684

Anti-EMA (CD227, Mucin-1)

DB-048-1 1 ml
EUR 450
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal Antibody

M00522-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal AFP/Alpha 1 Fetoprotein Antibody. Validated in IP, IF, WB and tested in Human.

Anti-SERPINA1/Alpha 1 Antitrypsin Rabbit Monoclonal Antibody

M00720-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal SERPINA1/Alpha 1 Antitrypsin Antibody. Validated in IP, IF, WB and tested in Human.
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